mouse anti-cdx2 clone 88  (Biocare Medical)

Journal: Cancers

Article Title: MicroRNA-9-5p-CDX2 Axis: A Useful Prognostic Biomarker for Patients with Stage II/III Colorectal Cancer

doi: 10.3390/cancers11121891

Figure Lengend Snippet: IHC examination of CDX2 in patients with stage II/III CRC: ( a ) Schematic representation of the workflow of immunohistochemistry of CDX2. ( b ) Expression of CDX2 in CRC specimens. Normal intestinal epithelial cells were used as an internal positive control. A Score 0 and B Score 0.5; were determined to be CDX2-negative. C Score 2 and D Score 3; were determined to be CDX2-positive. The scale bar represents 100 µm. ( c , d ) Kaplan–Meier curves for OS and RFS of the 174 patients with stage II/III CRC. CDX2: caudal-type homeobox transcription factor 2; CRC: colorectal cancer; OS: overall survival; RFS: relapse-free survival.

Article Snippet: Formalin-fixed, paraffin-embedded human colorectal tissues were stained with an anti-CDX2 mouse monoclonal antibody (Clone: CDX2-88, 1:50, BioGenex, San Ramon, CA, USA) using the avidin-biotin immunoperoxidase method.

Techniques: Immunohistochemistry, Expressing, Positive Control

Journal: Cancers

Article Title: MicroRNA-9-5p-CDX2 Axis: A Useful Prognostic Biomarker for Patients with Stage II/III Colorectal Cancer

doi: 10.3390/cancers11121891

Figure Lengend Snippet: Patient characteristics and CDX2 expression.

Article Snippet: Formalin-fixed, paraffin-embedded human colorectal tissues were stained with an anti-CDX2 mouse monoclonal antibody (Clone: CDX2-88, 1:50, BioGenex, San Ramon, CA, USA) using the avidin-biotin immunoperoxidase method.

Techniques: Expressing, Adjuvant

Journal: Cancers

Article Title: MicroRNA-9-5p-CDX2 Axis: A Useful Prognostic Biomarker for Patients with Stage II/III Colorectal Cancer

doi: 10.3390/cancers11121891

Figure Lengend Snippet: Univariate and multivariate analysis for RFS.

Article Snippet: Formalin-fixed, paraffin-embedded human colorectal tissues were stained with an anti-CDX2 mouse monoclonal antibody (Clone: CDX2-88, 1:50, BioGenex, San Ramon, CA, USA) using the avidin-biotin immunoperoxidase method.

Techniques: Significance Assay, Expressing, Adjuvant

Journal: Cancers

Article Title: MicroRNA-9-5p-CDX2 Axis: A Useful Prognostic Biomarker for Patients with Stage II/III Colorectal Cancer

doi: 10.3390/cancers11121891

Figure Lengend Snippet: Search for miRs that regulate CDX2 expression. ( a ) Representative flow cytometry plot. EpCAM + /FVD − /CDX2 low (1) and EpCAM + /FVD − /CDX2 high (2) tumor cells in the same sample were collected by flow cytometry. ( b ) Computational prediction of miRNAs capable of targeting CDX2; eight miRs predicted by at least two algorithms were selected. ( c ) Comparison of the relative miRNA levels of different miRs in FACS-isolated CDX2 high and CDX2 low tumor cells. (* p < 0.05).

Article Snippet: Formalin-fixed, paraffin-embedded human colorectal tissues were stained with an anti-CDX2 mouse monoclonal antibody (Clone: CDX2-88, 1:50, BioGenex, San Ramon, CA, USA) using the avidin-biotin immunoperoxidase method.

Techniques: Expressing, Flow Cytometry, Comparison, Isolation

Journal: Cancers

Article Title: MicroRNA-9-5p-CDX2 Axis: A Useful Prognostic Biomarker for Patients with Stage II/III Colorectal Cancer

doi: 10.3390/cancers11121891

Figure Lengend Snippet: ( a ) Schematic representation of the predicted miR-9-5p target site sequence within the 3′-UTR of CDX2 . Two nucleotides in the miR-9-5p sequence were mutated in the CDX2 mutant plasmid. The numbers indicate the position of nucleotides in the wild-type sequence of CDX2 (NM_001265). ( b ) The activity of the firefly luciferase gene which were inserted the CDX 2 3′-UTR and containing the target site for miR-9-5p. The data are presented as mean and SD of separate transfections ( n = 3, * p < 0.05). ( c ) Semi-quantitative RT-PCR analysis of CDX2 mRNA expression levels in SW480 cells showing the expression of CDX2 . ( d ) Suppression of the endogenous CDX2 protein in transient miR-9-5p-expressing SW480.

Article Snippet: Formalin-fixed, paraffin-embedded human colorectal tissues were stained with an anti-CDX2 mouse monoclonal antibody (Clone: CDX2-88, 1:50, BioGenex, San Ramon, CA, USA) using the avidin-biotin immunoperoxidase method.

Techniques: Sequencing, Mutagenesis, Plasmid Preparation, Activity Assay, Luciferase, Transfection, Quantitative RT-PCR, Expressing

Journal: Cancers

Article Title: MicroRNA-9-5p-CDX2 Axis: A Useful Prognostic Biomarker for Patients with Stage II/III Colorectal Cancer

doi: 10.3390/cancers11121891

Figure Lengend Snippet: Relationship of miR-9-1 and miR-9-5p. ( a ) Each sequence of pre-miR-9-1 and its mature RNAs miR-9-5p and miR-9-3p. ( b ) Relative expression of miR-9-5p in stable DLD-1-miR-9-1 and its control. ( c ) Suppression of the endogenous CDX2 protein both in stable DLD-1-miR-9-1 and transient miR-9-5p-expressing DLD-1.

Article Snippet: Formalin-fixed, paraffin-embedded human colorectal tissues were stained with an anti-CDX2 mouse monoclonal antibody (Clone: CDX2-88, 1:50, BioGenex, San Ramon, CA, USA) using the avidin-biotin immunoperoxidase method.

Techniques: Sequencing, Expressing, Control

Journal: Cancers

Article Title: MicroRNA-9-5p-CDX2 Axis: A Useful Prognostic Biomarker for Patients with Stage II/III Colorectal Cancer

doi: 10.3390/cancers11121891

Figure Lengend Snippet: Functional assays in DLD-1-miR-9-1 and pathological therapeutic effect in NAC/NACRT cases. ( a ) Cell proliferation assay in DLD-1-miR-9-1 ( n = 3, * p < 0.05). ( b ) Cell cycle assay in DLD-1-miR-9-1. Bar graph indicates the percentage of cells in G0/G1, S or G2/M phase ( n = 3, * p < 0.05). ( c ) Cytotoxicity assays in DLD-1-miR-9-1. Results are presented as the mean and SD ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001). ( d ) Relationship between CDX2 expression and pathological therapeutic effect of NAC/NACRT followed by surgical resection. NAC: neoadjuvant chemotherapy; NACRT: neoadjuvant chemoradiotherapy.

Article Snippet: Formalin-fixed, paraffin-embedded human colorectal tissues were stained with an anti-CDX2 mouse monoclonal antibody (Clone: CDX2-88, 1:50, BioGenex, San Ramon, CA, USA) using the avidin-biotin immunoperoxidase method.

Techniques: Functional Assay, Proliferation Assay, Cell Cycle Assay, Expressing